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目的：本研究利用组织芯片技术高通量地研究Fascin蛋白在食管癌中的表达。方法：采用56例食管癌病人的病理蜡块制作组织芯片,应用免疫组化的方法检测Fascin在食管癌组织中的表达情况。同时对8例食管癌标本的冰冻切片显微切割“纯”癌和非癌组织,进行蛋白印迹研究。结果：在55例可提供信息的病例中,总体上72．7%的肿瘤中为阳性染色。其中高分化阳性率为95．2%,中分化为75%,低分化为35．7%,Fascin表达与食管癌分化有关(P＜0．001)。蛋白印迹结果也证实在分化较好的食管癌中Fascin为高表达。结论：利用组织芯片可以应用于回顾性Fascin表达的研究,研究中发现食管癌中Fascin的表达与组织分化有关。该试验方法简便,易于在临床推广。
Objective: This study uses tissue microarray technology to study the expression of Fascin protein in esophageal cancer with high throughput. METHODS: Tissue microarrays were performed in 56 patients with esophageal cancer. The expression of Fascin in esophageal cancer tissues was detected by immunohistochemistry. At the same time, frozen sections of 8 esophageal cancer specimens were microdissected into “pure” cancerous and non-cancerous tissues and subjected to Western blotting. RESULTS: Of the 55 informative cases, 72.7% of the tumors were positively stained overall. The positive rate of high differentiation was 95.2%, moderate differentiation was 75%, and poor differentiation was 35.7%. The expression of Fascin was related to differentiation of esophageal cancer (P<0.001). Western blotting results also confirmed high expression of Fascin in well-differentiated esophageal cancer. Conclusion: The use of tissue microarrays can be applied to the study of retrospective Fascin expression. The study found that Fascin expression in esophageal cancer is related to tissue differentiation. The test method is simple and easy to promote in the clinic.