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目的了解广东省获得性胃肠炎患者中是否存在香港海鸥型菌(LH)感染。方法 2009年4月～12月选择江门市各级医院门诊腹泻患者,采集肛拭子标本,培养分离可疑菌株,经革兰染色、生化鉴定、特异性片段PCR扩增和16S rRNA基因序列测定,进行香港海鸥型菌的鉴定。结果在804例腹泻患者标本中,分离并鉴定1株香港海鸥型菌,符合已有报道菌的菌落特点和生化特征,命名为江门株(JM1),16S rRNA目的基因扩增长度为426 bp,基因序列长度为1 360 bp,与香港分离菌株(NR-025167)同源性为99%,确诊为香港海鸥型菌感染病例。结论证实广东省内社区获得性胃肠炎患者中存在香港海鸥型菌的感染。
Objective To understand whether there is a Hong Kong seagull (LH) infection in patients with acquired gastroenteritis in Guangdong Province. Methods From April 2009 to December 2009, patients with diarrhea in outpatient departments of hospitals in all levels of Jiangmen were selected. Samples of anal swabs were collected and suspicious strains were isolated. Gram stain, biochemical identification, PCR amplification of specific fragments and sequencing of 16S rRNA gene were performed. Hong Kong seagull-shaped bacteria identification. Results A total of 804 samples of diarrhea were isolated and identified as one type of seagull in Hong Kong, which accords with the characteristics of the colonies and biochemical characteristics of the reported bacteria. The strain was named Jiangmen strain (JM1). The length of 16S rRNA gene was 426 bp, The gene was 1 360 bp in length and 99% identical to Hong Kong isolated strain (NR-025167). It was diagnosed as a seagull infection in Hong Kong. Conclusion It is confirmed that there is a Hong Kong seagull-type infection in patients with community-acquired gastroenteritis in Guangdong Province.