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目的对潲水油的食用安全性进行毒理学观察。方法选取SD大鼠20只,按2.0 ml/100 g剂量实施一次性灌胃进行急性毒性实验;昆明种小鼠60只随机分为6组[受试物2.5、5.0、10.0 g/kg及正常对照组(生理盐水)、阴性对照组(食用油)、阳性对照组(环磷酰胺0.04 g/kg)]分阶段实施灌胃,30 h后处死小鼠取股骨的骨髓液制片,进行小鼠骨髓嗜多染红细胞微核实验;昆明种雄性小鼠30只随机分为6组[受试物2.5、5.0、10.0 g/kg及正常对照组(生理盐水)、阴性对照组(食用油)、阳性对照组(环磷酰胺0.04 g/kg)],连续灌胃5 d,实施观察35 d后,处死小鼠取双侧附睾制片,进行小鼠精子畸形实验;选取SD大鼠100只随机分为5组[受试物2.5、5.0、10.0 g/kg及正常对照组(生理盐水)、阴性对照组(食用油)]喂养30 d,观察其体质量变化和食物利用率,并检测其血常规及生化指标和病理改变。结果本次实验测定所用的潲水油急性毒性经口LD50大于10.0 g/kg。小鼠骨髓嗜多染红细胞微核实验为阳性,小鼠精子畸形实验为阴性。30 d喂养实验中,除低剂量潲水油外均可导致大鼠体质量增重减慢和食物利用率降低,高剂量潲水油可导致大鼠血清总蛋白和球蛋白含量降低,中、高剂量潲水油可导致大鼠肝脏系数增加及肝脏发生不同程度的病理改变。结论 本次实验所用的潲水油急性毒性实验为阴性结果,遗传毒性实验和30 d喂养实验为阳性结果。
Objective To study the toxicological effects of water consumption on the water quality. Methods Twenty SD rats were randomly divided into 6 groups ([2.5, 5.0, 10.0 g / kg] and normal group Control group (saline), negative control group (edible oil), positive control group (cyclophosphamide 0.04 g / kg)] were administered in different stages. After 30 h, the mice were sacrificed and bone marrow fluid preparation of the femur was performed. Thirty mice of Kunming male mice were randomly divided into 6 groups [test substance 2.5, 5.0, 10.0 g / kg and normal control group (normal saline), negative control group (edible oil) , The positive control group (cyclophosphamide 0.04 g / kg)], continuous gavage for 5 days, observed 35 days later, the mice were sacrificed to take bilateral epididymal preparations for sperm deformity test in mice; selected 100 SD rats The rats were randomly divided into five groups [test substance 2.5, 5.0, 10.0 g / kg and normal control group (normal saline), negative control group (edible oil]] for 30 days. The body weight and food utilization rate The blood and biochemical indicators and pathological changes. Results The acute toxicity of the water used in this experiment was determined by oral LD50 greater than 10.0 g / kg. Mouse bone marrow polychromatic erythrocyte micronucleus test was positive, mouse sperm deformity test was negative. In the 30-day feeding experiment, body weight gain and food utilization rate decreased except for the low dose of water, while the high dose of water oil caused the decrease of serum total protein and globulin, Sui water oil can lead to increased liver coefficient in rats and the liver to varying degrees of pathological changes. Conclusion The acute toxicity test of the emu water used in this experiment is a negative result. The genotoxicity test and the 30 d feeding test are positive results.