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目的 :探讨结肠正常组织和腺癌组织的显微自体荧光图像差异与肠壁不同结构层次的荧光强度差异。方法 :采用共聚焦显微自体荧光图像系统采集与分析结肠正常和腺癌组织的自体荧光图像。结果 :结肠正常组织自体荧光图像能清楚显示肠壁的三层结构 ,自体荧光主要来自于细胞间质和粘膜下层 ,上皮细胞的荧光强度很弱 ;腺癌组织的肠壁结构发生明显改变 ,荧光强度主要来自于粘膜下层 ;腺癌组织的显微自体荧光强度显著低于正常组织 (P <0 .0 5 ) ,表明自体荧光分析法用于大肠癌的诊断是一个可行的方法。
Objective : To investigate the differences of microscopic autofluorescence images between normal colon tissue and adenocarcinoma tissue and the differences of fluorescence intensity at different structural layers of the intestinal wall. Methods: Autofluorescence images of normal and adenocarcinoma of colon were collected and analyzed by confocal microscopy autofluorescence imaging system. RESULTS: Autofluorescence images of normal colon tissues clearly showed the three-layer structure of the intestinal wall. Autofluorescence mainly came from the interstitial and submucosa. The fluorescence intensity of the epithelial cells was very weak; the intestinal wall structure of the adenocarcinoma tissue changed significantly. The intensity mainly comes from the submucosa; the autofluorescence intensity of adenocarcinoma tissue is significantly lower than that of normal tissue (P < 0.05), indicating that autofluorescence assay is a feasible method for the diagnosis of colorectal cancer.