日本脑炎病毒 (ＪＥＶ)为黄病毒属成员 ,基因组为单股正链ＲＮＡ ,长约 11ｋｂ ,编码 3个结构蛋白和7个非结构蛋白〔1〕。其中 3个结构蛋白分别为衣壳蛋白Ｃ、膜蛋白Ｍ和囊膜蛋白Ｅ。Ｅ蛋白为该病毒外部的糖蛋白 ,由 50 0个氨基酸组成 ,相对分子质量(Ｍｒ)约为 530 0 0 ,具有多 Japanese encephalitis virus (JEV) is a member of the genus Flaviviridae. The genome of the Japanese encephalitis virus (JEV) is a single-stranded positive-sense RNA of about 11 kb encoding three structural proteins and seven nonstructural proteins [1]. Three of the structural proteins were capsid protein C, membrane protein M and envelope protein E. The E protein is a glycoprotein outside the virus consisting of 50 0 amino acids and has a relative molecular mass (Mr) of about 530 0, with more