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目的：研究碱性成纤维细胞生长因子（ｂＦＧＦ）对大鼠脑缺血再灌注损伤的影响．方法：电灼闭塞椎动脉并夹闭颈动脉，使大鼠前脑缺血２０分钟后，放开双侧颈动脉再灌．用高压液相色谱法测定纹状体中多巴胺（ＤＡ）类物质含量并用原子吸收分光光度法测定前脑中钠、钾含量．结果：再灌６小时后，纹状体中ＤＡ含量由对照的（９９±１６）μｇ·ｇ－１（蛋白）减少至（７０±２０）μｇ·ｇ－１蛋白；脑含水量由７７３４％±０１９％增加到７９６％±０６％；钠含量由（９３±０６）ｍｇ·ｇ－１（脑干重）增加到（１０５±０６）ｍｇ·ｇ－１（脑干重）．再灌开始后即刻静注ｂＦＧＦ４５μｇ·ｋｇ－１·ｈ－１共３ｈ可阻止ＤＡ的减少和水、钠含量的升高．组织学分析也表明ｂＦＧＦ可减轻神经元所受损伤．结论：ｂＦＧＦ可以保护神经细胞对抗脑缺血再灌损伤．
Objective: To investigate the effect of basic fibroblast growth factor (bFGF) on cerebral ischemia-reperfusion injury in rats. Methods: The vertebral artery was occluded and the carotid artery was occluded by electrocautery. After 20 min of ischemia in the forebrain, the bilateral carotid artery was released. The levels of dopamine (DA) in striatum were determined by high pressure liquid chromatography and the contents of sodium and potassium in the forebrain were determined by atomic absorption spectrophotometry. Results: After 6 hours of reperfusion, the content of DA in the striatum decreased from (99 ± 16) μg · g-1 (protein) to (70 ± 20) μg · g-1 protein in the striatum; 34 ± 019% to 796% ± 06%, while the sodium content increased from (93 ± 06) mg · g-1 (brain stem weight) to (105 ± 0 6) mg · g -1 (brainstem weight). Immediate infusion of bFGF 45μg · kg-1 · h-1 for 3 hours after initiation of reperfusion prevented the decrease of DA and the increase of sodium and water. Histological analysis also showed that bFGF can reduce neuronal damage. Conclusion: bFGF can protect nerve cells against cerebral ischemia-reperfusion injury.