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近来研究表明结节病患者BALF 中淋巴细胞数增高,皮质类甾醇对结节病的治疗反应,可能依赖于BALF 细胞的糖皮质激素受体(GR)的含量。为了弄清患者灌洗液中GR 含量与糖皮质激素治疗反应间的关系,作者测定了20例未治疗的结节病患者BALF细胞中GR 含量,并以10例正常志愿者为对照。GR 测定用三层纱布过滤BALF,滤液离心(200Xg,4(?)C,10min),沉淀细胞用生理盐水洗2次,悬于无或有4×10~(-(?))M 未标记的丙酮去炎松的磷酸缓冲液PBS 中,悬液同4×10~(-8)M~3 H 丙酮去炎松(39Ci/mm01ol)一起在4℃轻轻摇动孵
Recent studies have shown that lymphocytes in BALF in patients with sarcoidosis increased, corticosteroid treatment of sarcoidosis response may depend on the content of glucocorticoid receptor (GR) in BALF cells. In order to clarify the relationship between GR content in patients’ lavage fluid and glucocorticoid response, the authors determined the GR content of BALF cells in 20 untreated sarcoidosis patients and compared them to 10 normal volunteers. GR assay The BALF was filtered through a three-layer gauze and the filtrate was centrifuged (200 × g, 4 (?) C, 10 min). The pelleted cells were washed twice with normal saline and suspended without or with 4 × 10 ~ (- Of acetone to the phosphate buffer PBS, the suspension with 4 × 10 ~ (-8) M ~ 3 H acetone triamcinolone (39Ci / mm01ol) together at 4 ℃ gentle shaking incubation