论文部分内容阅读
目的 分析深圳地区阴道加德纳菌 (G .vag)ITS(internaltranscribedspacer) 2 3SrRNA部分基因序列。方法 设计特异性引物 ,建立检测G .vag的PCR方法 ,对临床病人阴道分泌物标本进行检测 ,并对分离出的 4株PCR产物进行分子克隆和序列分析。结果 分离出的 4株G .vagITS 2 3SrRNA基因的核苷酸同源性在 99 5 %以上 ,与Belkum报道株同源性在 98%以上。结论 深圳地区G .vag与国外报道的菌株在ITS 2 3SrRNA基因区变异不大 ,可能属同一基因型。
Objective To analyze the partial gene sequence of 23SrRNA of G. vaginalis ITS (internaltranscribedspacer) 2 in Shenzhen. Methods Specific primers were designed and a PCR method was established to detect Gvag. The specimens of vaginal secretions from clinical patients were detected. The 4 PCR products were cloned and sequenced. Results The nucleotide homology of the four strains of .vagITS 2 3SrRNA isolated in the study was over 99.5%. The nucleotide homology was more than 98% with that reported by Belkum. Conclusion The strains of G.vag in Shenzhen and those reported abroad are not significantly different in the ITS2 3SrRNA gene region, which may belong to the same genotype.