论文部分内容阅读
目的:筛选青果中抗炎镇痛的活性部位,探讨活性部位对细胞炎性因子IL-1β、IL-10、TNF-α蛋白表达的影响。方法:分别用水蒸气蒸馏法、溶剂梯度萃取法获得青果挥发油、青果10%水提液及其石油醚、氯仿、乙酸乙酯、正丁醇萃取部位。首先采用二甲苯致小鼠耳廓肿胀、角叉菜胶致大鼠足跖肿、醋酸致扭体反应和小鼠热板性疼痛筛选抗炎镇痛的活性部位,然后采用Western blot检测活性部位对炎性因子IL-1β、IL-10、TNF-α蛋白表达的影响。结果:青果水提液及其石油醚、氯仿、乙酸乙酯萃取部位800mg/kg均能减轻二甲苯致小鼠耳廓肿胀和角叉菜胶致大鼠足跖肿胀,提高小鼠对热板性疼痛的痛阈值,减少扭体反应的平均次数,其中乙酸乙酯萃取部位活性最强,但青果挥发油和正丁醇萃取部位800 mg/kg无明显抗炎镇痛作用。青果水提液的乙酸乙酯萃取部位400 mg/kg、800 mg/kg、1200 mg/kg显著降低角叉菜胶致足跖肿胀模型大鼠细胞因子IL-1β和TNF-α的蛋白表达,但对IL-10表达无明显的影响。结论:青果水提液的乙酸乙酯萃取部位是抗炎镇痛的活性部位,其作用机制可能与抑制细胞炎性因子IL-1β和TNF-α的表达有关。
Objective: To screen anti-inflammatory and analgesic active sites of Qingguo and explore the effect of active site on the expression of inflammatory cytokines IL-1β, IL-10 and TNF-α. Methods: Volatile oil of Vitis vinifera, extract of green fruit 10% and petroleum ether, chloroform, ethyl acetate and n-butanol were extracted by steam distillation and solvent gradient extraction respectively. First of all, xylene-induced mouse ear swelling, carrageenan-induced rat paw edema, acetic acid induced writhing reaction and mouse hot plate pain screening anti-inflammatory analgesic active site, and then using Western blot detection of active site On the inflammatory factors IL-1β, IL-10, TNF-α protein expression. Results: The extract of Qingguo and petroleum ether, chloroform and ethyl acetate extract 800mg / kg can reduce xylene-induced mouse ear swelling and carrageenan induced paw edema in rats to improve the hot plate to mice The pain threshold of pain and the average number of writhing reactions were reduced. Among them, the ethyl acetate extract had the strongest activity, but there was no obvious anti-inflammatory and analgesic effect at 800 mg / kg of volatile oil and n-butanol extract. The ethyl acetate extracts 400mg / kg, 800 mg / kg and 1200 mg / kg of the aqueous extract of P. chinensis significantly reduced the protein expressions of IL-1β and TNF-α in rats with paw edema induced by carrageenan, But had no obvious effect on IL-10 expression. CONCLUSION: The ethyl acetate fraction extracted from Fructus Chinensis extract is an active site of anti-inflammatory and analgesic activity. Its mechanism may be related to the inhibition of the expression of inflammatory cytokines IL-1β and TNF-α.