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DNA甲基化作为一种重要的表观遗传修饰,广泛存在于高等动植物中,并在维持基因组稳定性、调节基因表达等方面起着重要作用,因此建立快速有效地DNA甲基化检测技术至关重要。本文以两种不同MuDR活性的玉米转座子材料为研究对象,探讨了甲基化特异性PCR(MSP)在检测DNA甲基化的有效性。结果表明:MSP技术可快速有效地检测MuDR转座子的末端反向重复(TIRs)序列内的CpG岛DNA甲基化的变化,灵敏度高,特异性强,可作为植物已知基因DNA甲基化检测的一种新方法。同时利用MSP研究发现,玉米MuDR转座子的活性随其TIRs序列内的CpG岛DNA甲基化的变化而改变,DNA甲基化是调控玉米MuDR转座活性的重要分子机制之一。
As an important epigenetic modification, DNA methylation plays an important role in higher plants and animals and plays an important role in maintaining genome stability and regulating gene expression. Therefore, DNA methylation assay has been established to detect DNA methylation rapidly and efficiently It is very important. In this paper, two kinds of maize transposable material with different activity of MuDR were studied to investigate the effectiveness of methylation-specific PCR (MSP) in detecting DNA methylation. The results showed that MSP could detect DNA methylation changes of CpG islands in the terminal inverted repeat (TIRs) sequence of MuDR transposon quickly and efficiently with high sensitivity and specificity and could be used as DNA methyl A new method of testing. At the same time using MSP found that the activity of MuDR transposon in maize changes with the DNA methylation of CpG island in TIRs sequence. DNA methylation is one of the important molecular mechanisms that regulate the transposition activity of MuDR in maize.