论文部分内容阅读
目的:探讨NK细胞在重度子痫前期发病中的作用及其机制。方法:采集重度子痫前期晚孕、正常晚孕孕妇外周血及其新生儿脐血,PERCOLL分离NK细胞,SAP法计数,MTT法检测其增殖活性,[51CR]释放法检测其杀伤活性,免疫组织化学法检测胎盘蜕膜组织NK细胞数量及HLA-G蛋白表达水平。结果:①重度子痫前期患者外周血、蜕膜组织及其新生儿脐血中NK细胞数量均显著高于正常晚孕组(P<0．05);②重度子痫前期患者外周血及其新生儿脐血NK细胞增殖和杀伤活性均高于正常晚孕组(P<0．05);③重度子痫前期患者胎盘蜕膜HLA-G蛋白表达水平显著低于正常晚孕组(P<0．05)。结论:重度子痫前期患者NK细胞功能异常增强,可能参与疾病发生。
Objective: To investigate the role of NK cells in the pathogenesis of severe preeclampsia and its mechanism. Methods: Peripheral blood and cord blood were collected from pregnant women with severe preeclampsia, normal pregnant women with umbilical cord blood and PERCOLL isolated from normal pregnant women. The proliferative activity was detected by MTT assay and the cytotoxicity was measured by [51CR] release assay. Tissue chemistry was used to detect the number of NK cells and HLA-G protein expression in placental decidual tissue. Results: (1) The number of NK cells in peripheral blood, decidua and umbilical blood in patients with severe preeclampsia were significantly higher than those in normal pregnant women (P <0.05); ②The levels of NK cells in peripheral blood of patients with severe preeclampsia Neonatal cord blood NK cell proliferation and killing activity were higher than normal late pregnancy group (P <0.05); ③ severe preeclampsia placental expression of HLA-G protein was significantly lower than the normal pregnancy group (P < 0.05). Conclusion: Patients with severe preeclampsia NK cell dysfunction increased, may be involved in the disease.