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为了研究在肠道阻塞的病理条件下,血浆蛋白质的变化及其在诊断、治疗和判断预后上的作用进行此项工作。材料和方法本项试验与牛急腹症的研究Ⅰ、Ⅱ、Ⅲ同时进行。血清总蛋白、白蛋白、球蛋白用双缩脲法测定。血清蛋白电泳采用pH8.5、离子强度为0.06的巴比妥和巴比妥钠缓冲液,以醋酸纤维薄膜为支持物,电泳端电压为110V,通电时间50—60分钟,常规染色,漂洗和比色测定。
In order to study the changes of plasma proteins in the pathological conditions of intestinal obstruction and its role in the diagnosis, treatment and prognosis of this disease. Materials and Methods This study and the study of bovine acute abdomen Ⅰ, Ⅱ, Ⅲ at the same time. Serum total protein, albumin, globulin measured by the biuret method. Serum protein electrophoresis using pH8.5, ionic strength of 0.06 barbital and barbital sodium buffer to acetate film as a support, electrophoresis voltage was 110V, power time 50-60 minutes, conventional dyeing, rinsing and Colorimetric determination.