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目的通过新的维吾尔族妇女宫颈癌组织特异性上调表达基因的分析,探讨宫颈癌发生与基因表达调控的关系及临床意义。方法选择6种新发现的宫颈癌组织特异性上调表达候选基因,设计mRNA特异性引物,对25例宫颈鳞癌(cervical squamous cell carcinoma,CSCC)、22例宫颈内上皮瘤变(cervical intraepithelial neoplasia,CIN)Ⅱ~Ⅲ和25例宫颈炎组织RNA进行半定量RT-PCR鉴定。结果 CSCC与宫颈炎组织比较,SHISA2、SIX1、果蝇同源盒基因(DTL)、桥粒芯糖蛋白-2(DSG2)、NUP62CL和胞嘧啶脱氨酶(APOBE63B)6种基因的mRNA水平均有差异(P<0.05);CSCC与CINⅡ~Ⅲ比较,SHISA2、SIX1、APOBEC3B和DSG2 4种基因的mRNA水平有差异(P<0.05),而CINⅡ~Ⅲ与宫颈炎比较,只有SHISA2基因的mRNA水平差异有统计学意义(P<0.05)。结论 SHISA2、SIX1、DTL、DSG2、NUP62CL和APOBE63B6种基因的转录表达水平变化可能成为维吾尔族妇女宫颈癌发生的早期预警标志物。
Objective To investigate the relationship between the occurrence of cervical cancer and the regulation of gene expression and its clinical significance through the new Uyghur cervical cancer tissue-specific up-regulated gene expression analysis. Methods Six kinds of newly discovered cervical cancer tissues were selected to up-regulate the expression of candidate genes. MRNA specific primers were designed, and 25 cases of cervical squamous cell carcinoma (CSCC), 22 cases of cervical intraepithelial neoplasia CIN) Ⅱ ~ Ⅲ and 25 cases of cervicitis tissue RNA semi-quantitative RT-PCR identification. Results Compared with cervicitis tissues, the mRNA levels of six genes of SHISA2, SIX1, Drosophila homologous box gene (DTL), desmoglein-2 (DSG2), NUP62CL and cytosine deaminase (APOBE63B) (P <0.05). Compared with CINⅡ ~ Ⅲ, CSCC mRNA levels of four genes of SHISA2, SIX1, APOBEC3B and DSG2 were significantly different (P <0.05), while only mRNA of SHISA2 gene The difference was statistically significant (P <0.05). Conclusion The transcriptional expression of SHISA2, SIX1, DTL, DSG2, NUP62CL and APOBE63B may be the early warning markers for cervical cancer in Uighur women.