目的 :探讨创伤性休克时白细胞表面淋巴细胞功能相关抗原 1(LFA 1) ,细胞内粘附分子 (ICAM 1)的表达变化。方法 :以创伤休克大鼠为模型 ,用单克隆抗体间接免疫荧光标记法测定休克时白细胞表面LFA 1,ICAM 1的表达。结果 :创伤后 3h测得的多形核粒细胞表面LFA 1,ICAM 1表达量均明显降低 (P <0 0 5 )。而单核白细胞表面LFA 1,ICAM 1表达量却无变化。结论 :LFA 1/ICAM 1依赖的白细胞粘附机制不是剂量依赖性的 ,ICAM 1未见增多可能与其白细胞膜脱落有关。进一步研究LFA 1,ICAM 1功能结构改变和其它粘附分子变化 ,将有利于阐明创伤性休克时白细胞粘附机制 Objective: To investigate the expression of LFA 1 and ICAM 1 on leukocyte surface during traumatic shock. Methods: The expression of LFA 1 and ICAM 1 on the surface of leukocytes was measured by indirect immunofluorescent labeling with the method of traumatic shock rat model. Results: The expression of LFA 1 and ICAM 1 on the surface of polymorphonuclear neutrophils was significantly decreased 3 h after trauma (P <0.05). However, the expression of LFA 1 and ICAM 1 on the surface of mononuclear leucocytes did not change. CONCLUSION: LFA 1 / ICAM 1-dependent leukocyte adhesion mechanism is not dose-dependent, and no increase of ICAM 1 may be related to its leukocyte membrane detachment. Further study of LFA 1, ICAM 1 functional structure changes and other changes in adhesion molecules, will help elucidate the traumatic shock leukocyte adhesion mechanism