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后基因组时代涌现出DNA操作技术一系列新的应用,其中包括建立在DNA分子基础上的生物计算机.至今还没有在固体表面实现基于自动机原理的DNA计算的相关报道.本文描述了一种可编程的DNA计算装置——具有图灵机部分功能的有限自动机,并且将计算过程转移到了固体表面.DNA计算机主要由DNA分子(输入分子,输出状态检测分子和充当软件的状态转移分子等)、DNA限制性内切酶及连接酶和所需的缓冲液组成,可以自动地解决计算问题.将荧光标记在输入分子的5’端,以便于动态跟踪监测反应过程中的各种中间产物.这些工作具有如下的特点:(1)成功地在磁珠表面实现了可编程的DNA计算机——有限状态自动机.(2)通过毛细管电泳直接监测所有的DNA计算中间产物.DNA计算机的超大并行计算能力具有通过自动控制来得以实现的可行性,为在不久的将来把大规模的DNA计算和功能基因组研究结合起来奠定了基础.
Post-genome era emerged a series of new applications of DNA manipulation technology, including biological computer based on DNA molecules.There has been no report on the basis of automata principle of DNA calculation on solid surface.This paper describes a The programmed DNA computing device - a finite automaton with some of the functions of the Turing machine - transfers the computational process to a solid surface.The DNA computer consists of DNA molecules (input molecules, output state detection molecules and state transition molecules acting as software, etc.) DNA restriction enzymes and ligases and the required buffers to automatically solve computational problems Fluorescence is labeled at the 5 ’end of the input molecule to allow for dynamic follow-up monitoring of various intermediates during the reaction These The work has the following characteristics: (1) Successful realization of a programmable DNA computer - finite state automaton on the bead surface (2) Direct monitoring of all DNA by capillary electrophoresis to calculate intermediate products. Capabilities have the potential to be achieved through automated control, and in the near future to bring large-scale DNA computing and functionality Genomic research combined basis.