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目的:通过比较广西北回归线两侧的南宁地区和桂林地区人群肝组织黄曲霉毒素B1(Aflatoxin B1,AFB1)的暴露水平,探讨AFB1暴露与原发性肝细胞癌(Hepatocellular carcinoma,HCC)发生的关系。方法:应用免疫组化法检测南宁和桂林地区共165例HCC患者和95例正常肝组织中AFB1-DNA加合物的表达水平。结果:AFB1-DNA加合物阳性率分别为:南宁和桂林地区HCC组分别为59.5%(47/79)与17.4%(15/86)(χ2=31.042,P<0.001);南宁与桂林地区对照组分别为15.5%(9/58)和10.8%(4/37)(χ2=1.632,P=0.121);南宁地区HCC组59.5%(47/79)与对照组15.5%(9/58)间阳性率差异有统计学意义(χ2=21.484,P<0.001),桂林地区HCC组17.4%(15/86)与对照组10.8%(4/37)间阳性率无统计学差异(χ2=0.318,P=0.573)。结论:广西AFB1暴露水平存在区域性差异,南宁地区AFB1暴露水平要远高于桂林地区。AFB1暴露是南宁地区HCC高发的危险因素。
Objective: To investigate the relationship between AFB1 exposure and the occurrence of primary hepatocellular carcinoma (HCC) by comparing the levels of aflatoxin B1 (AFB1) in liver tissue of Nanning and Guilin areas on both sides of the Tropic of Cancer relationship. Methods: The expression of AFB1-DNA adduct in 165 HCC patients and 95 normal liver tissues from Nanning and Guilin was detected by immunohistochemistry. Results: The positive rates of AFB1-DNA adduct were 59.5% (47/79) and 17.4% (15/86) in the HCC group (χ2 = 31.042, P <0.001) in Nanning and Guilin areas, respectively; In the control group, 15.5% (9/58) and 10.8% (4/37) in the control group (χ2 = 1.632, P = 0.121) (Χ2 = 21.484, P <0.001). The positive rates of 17.4% (15/86) in HCC group and 10.8% (4/37) in control group were not statistically different (χ2 = 0.318 , P = 0.573). Conclusion: There is a regional difference in the level of AFB1 exposure in Guangxi. The level of AFB1 exposure in Nanning is much higher than that in Guilin. AFB1 exposure is a high risk factor for HCC in Nanning.