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目的 观察野生型 P53基因对前列腺癌细胞系 DU1 4 5致瘤性的抑制作用。方法 将野生型 P53真核表达载体用 lipofec-tamine导入 DU1 4 5细胞中。PCR法和抗 P53基因单抗免疫组化方法检测 P53基因的表达情况 ,MTT法检测细胞的生长程度 ,流式细胞仪检测细胞的凋亡情况 ,软琼脂生长试验检测细胞集落形成能力的变化。结果 P53基因可转染入 DU1 4 5细胞中 ,且对其致瘤性有一定的抑制作用。结论 本实验从理论上证明了利用野生型 P53基因对前列腺癌进行基因治疗的可行性。
Objective To observe the inhibitory effect of wild-type P53 gene on the tumorigenicity of prostate cancer cell line DU145. Methods The wild type P53 eukaryotic expression vector was introduced into DU145 cells with lipofec-tamine. PCR and anti-P53 monoclonal antibody immunohistochemistry method were used to detect the expression of P53 gene, MTT assay was used to detect the cell growth, flow cytometry was used to detect cell apoptosis, and soft agar growth test was used to detect the change of cell colony formation ability. Results P53 gene was transfected into DU145 cells, and its tumorigenicity was inhibited. Conclusion This experiment demonstrated theoretically the feasibility of using wild-type P53 gene for gene therapy of prostate cancer.