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目的探讨广西地区未感染艾滋病病毒(HIV)婴儿T淋巴细胞及亚群的基本特征,为初步排除并辅助诊断HIV阳性产妇所生婴儿是否感染HIV提供依据。方法收集68例未感染HIV婴儿(未感染组)和13例感染HIV婴儿(对照组)的抗凝全血,用四色荧光流式细胞术检测外周血CD3+、CD4+、CD8+T淋巴细胞绝对数及其百分比,并计算CD4/CD8比值;用Nuclisens EasyQ方法检测血浆HIV-1病毒载量。结果 (1)未感染组外周血CD3+、CD4+、CD8+T淋巴细胞绝对数(细胞/mm3)的中位数分别为4 5702、8181、410;CD3、CD4、CD8的百分比中位数分别为61.42%、39.39%、18.40%;CD4/CD8比值的中位数为2.25;在未感染组中,男性婴儿与女性婴儿的T淋巴细胞及亚群的比较,各检测指标差异没有统计学意义(P>0.05);血浆HIV-1病毒载量均为未检测出水平。(2)对照组外周血CD3+、CD4+、CD8+T淋巴细胞绝对数的中位数分别为4 930、1 4603、326(细胞/mm3);CD3、CD4、CD8百分比的中位数分别为69.95%、13.77%、54.69%;CD4/CD8比值的中位数为0.25;血浆HIV-1病毒载量的中位数为800 000(拷贝/ml)。(3)除CD3+T淋巴细胞绝对数之外,未感染组与对照组的CD4+、CD8+T淋巴细胞绝对数、CD3、CD4、CD8百分比及CD4/CD8比值的比较,差异有统计学意义(P<0.05)。结论在资源有限的情况下,参考婴儿T淋巴细胞及亚群的异常变化,可初步判断HIV阳性产妇所生婴儿是否感染HIV,T淋巴细胞及亚群的检测,可以作为一种辅助诊断HIV感染的手段。
Objective To investigate the basic characteristics of T lymphocytes and their subpopulations in infants without HIV in Guangxi and provide the basis for the preliminary exclusion and diagnosis of HIV in infants born to HIV-positive mothers. Methods The anticoagulant whole blood of 68 infants without HIV (uninfected group) and 13 infants infected with HIV (control group) were collected. The absolute levels of CD3 +, CD4 +, CD8 + T lymphocytes in peripheral blood were detected by four-color fluorescence flow cytometry CD4 + CD8 ratio and plasma HIV-1 viral load by Nuclisens EasyQ method. Results (1) The median number of CD3 +, CD4 +, CD8 + T lymphocytes in non-infected group was 4 5702, 8181, and 410 respectively. The median percentage of CD3, CD4 and CD8 were 61.42%, 39.39% and 18.40%, respectively. The median CD4 / CD8 ratio was 2.25. There was no significant difference in T lymphocytes and subpopulations between male and female infants in uninfected group P> 0.05). The plasma HIV-1 viral load was undetectable. (2) The median absolute numbers of CD3 +, CD4 + and CD8 + T lymphocytes in peripheral blood of the control group were 4930, 14603 and 326 (cells / mm3), respectively. The median percentage of CD3, CD4 and CD8 were 69.95 %, 13.77%, 54.69% respectively. The median CD4 / CD8 ratio was 0.25. The median HIV-1 viral load in plasma was 800 000 copies / ml. (3) In addition to the absolute number of CD3 + T lymphocytes, the absolute number of CD4 +, CD8 + T lymphocytes, CD3, CD4, CD8 percentage and CD4 / CD8 ratio of non-infected group and control group, the difference was statistically significant (P <0.05). Conclusions With limited resources, referring to the abnormal changes of T lymphocytes and subpopulations in infants, we can initially determine whether the infants born to HIV-positive mothers are infected with HIV or T-lymphocytes or subpopulations, which can be used as a diagnosis of HIV infection s method.