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采用等电聚焦凝胶电泳比较了抗、感白粉病小麦品种的过氧化物酶同工酶酶谱，发现抗、感品种之间的叶片过氧化物酶同工酶ｐＩ６．１酶带（位于过氧化物酶同工酶等电聚焦凝胶电泳酶谱ｐＩ６．１）在拔节期至白粉病发生期表现水平有很大差别。抗病品种中编码ｐＩ６．１酶带的基因终生正常表达，该酶带在小麦全生育期均有较强的活性，染色后着色深，多属一级酶带。而该基因的表达在感病品种拔节之后受到抑制，酶带变得模糊不清甚至消失踪迹，多为三级、零级酶带。感染白粉病之后酶活性才回升，再度表现为一、二级酶带。为了探索造成这一差异的原因，比较了１１９株抗病品种与感品种杂交Ｆ２代和６５株抗病品种／感病品种／／感病品种的ＢＣ１代植株叶片过氧化物酶同工酶ＩＥＦ酶谱，证明感病品种成株的ｐＩ６．１酶带活性下降是因另一隐性基因所至。
By isoelectric focusing gel electrophoresis, the peroxidase isozymes of wheat cultivars with resistant and susceptible powdery mildew were compared. It was found that the isozymes of the peroxidase isozymes pI6.1 Peroxidase isoenzyme isoelectric focusing gel electrophoresis pI6.1) at the jointing stage to the occurrence of powdery mildew in the performance level is very different. The genes encoding pI6.1 in resistant cultivars were all expressed for a lifetime, with strong activity throughout the whole wheat growing period. However, the expression of this gene was inhibited after the susceptible variety was jointed, and the enzyme band became obscure or even disappeared. Infected with powdery mildew enzyme activity was only picked up again for the performance of a secondary enzyme band. In order to explore the reasons for this difference, we compared the leaf peroxidase isozymes IEF of 119 resistant and susceptible F2 generation and 65 resistant / susceptible / susceptible BC1 plants Zymogram to prove susceptible varieties of adult pI6.1 enzyme activity decreased due to another recessive gene.