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目的探讨凋亡抑制基因Bcl-2与血管内皮生长因子(VEGF)蛋白在宫颈鳞状细胞癌中的表达,以及二者表达与宫颈鳞状细胞癌中生物学行为的相关性。方法分别对45例宫颈鳞状细胞癌中组织、34例CIN组织和10例正常宫颈组织,采用免疫组织化学法检测Bcl-2的表达,免疫蛋白印迹(Western Blotting)法检测VEGF蛋白的表达,并采用DNA原位末端标记(TUNEL)法检测细胞凋亡指数(AI)。结果 Bcl-2和VEGF蛋白在45例宫颈鳞状细胞癌组织中的阳性表达率分别为51.11%和62.22%,均显著高于CIN组织(11.76%和5.88%)(P<0.05);而在正常宫颈组织二者均无表达。同时,Bcl-2在VEGF表达阳性的宫颈鳞状细胞癌组织中呈高度表达,Bcl-2和VEGF的表达密切相关(χ2=5.148,P<0.05)。结论提示Bcl-2可能参与宫颈鳞状细胞癌的发生和发展;Bcl-2可能通过上调VEGF的表达而促进宫颈鳞状细胞癌的转移,同时VEGF也可能通过诱导Bcl-2的表达而抑制肿瘤细胞的凋亡。
Objective To investigate the expression of apoptosis-related genes Bcl-2 and VEGF in cervical squamous cell carcinoma and their correlation with the biological behavior of cervical squamous cell carcinoma. Methods 45 cases of squamous cell carcinoma of the cervix, 34 cases of CIN and 10 cases of normal cervical tissue were detected by immunohistochemical method. The expression of VEGF protein was detected by Western Blotting. Apoptosis index (AI) was detected by TUNEL method. Results The positive rates of Bcl-2 and VEGF in 45 cases of cervical squamous cell carcinoma were 51.11% and 62.22%, respectively, which were significantly higher than those in CIN tissues (11.76% and 5.88%, P <0.05) Neither normal cervical tissue expression. Meanwhile, Bcl-2 was highly expressed in VEGF-positive cervical squamous cell carcinoma tissue, and the expression of Bcl-2 and VEGF was closely related (χ2 = 5.148, P <0.05). Conclusion Bcl-2 may be involved in the occurrence and development of cervical squamous cell carcinoma. Bcl-2 may promote the metastasis of cervical squamous cell carcinoma by upregulating the expression of VEGF, and VEGF may also inhibit the tumor by inducing Bcl-2 expression Apoptosis of cells.