论文部分内容阅读
目的研究旋毛虫肌幼虫排泄分泌蛋白对H446小细胞肺癌的抑制作用。方法试验随机分为3组。A组(实验组):将提取的旋毛虫肌幼虫排泄分泌蛋白用无血清培养基稀释成浓度为0.075、0.15和0.3mg/ml,分别与H446细胞共培养;B组(标准对照):将顺铂稀释成12.8、6.4、3.2、1.6、0.8、0.4和0.2μg/ml,分别与H446细胞共培养;C组(空白对照组):H446细胞培养过程中不作任何处理。采用噻唑蓝比色法(MTT法)检测各组不同时间细胞抑制率;采用原位末端转移酶标记法(TUNEL法)观察细胞凋亡情况。结果 MTT法检测各实验组细胞抑制率均随药物浓度的增高和作用时间的延长而升高,与空白对照组比较差异有统计学意义(P<0.01);TUNEL法检测A、B、C组细胞凋亡率分别为85%、43%和2%,差异有统计学意义(P<0.01);A组与B组比较差异有统计学意义(P<0.01)。结论旋毛虫肌幼虫排泄分泌蛋白对H446细胞有抑制作用,可能是潜在的抗小细胞肺癌的生物制剂。
Objective To study the inhibitory effect of excretory protein secreted by Trichinella spiralis muscle larvae on H446 small cell lung cancer. Methods Tests were randomly divided into three groups. Group A (experimental group): the extracted Trichinella spiralis muscle larvae excreted secretory protein diluted with serum-free medium to a concentration of 0.075,0.15 and 0.3mg / ml, were co-cultured with H446 cells; Group B (standard control): Cisplatin was diluted to 12.8, 6.4, 3.2, 1.6, 0.8, 0.4 and 0.2 μg / ml, respectively, and co-cultured with H446 cells. Group C (blank control): H446 cells were cultured without any treatment. Cell inhibition rate was measured by MTT assay at different time points. Apoptosis was observed by TUNEL method. Results MTT assay showed that the inhibitory rate of cells in each experimental group increased with the increase of drug concentration and prolongation of action time, which was significantly different from that of the blank control group (P <0.01); TUNEL assay of A, B and C groups The apoptotic rate was 85%, 43% and 2%, respectively, with significant difference (P <0.01). There was significant difference between group A and group B (P <0.01). Conclusion Trichinella spiralis muscle larvae excreted secretory protein can inhibit H446 cells and may be potential biological agents for anti-small cell lung cancer.