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采用反转录ＰＣＲ方法，以一对锚ＰＣＲ引物和一对针对鼠ＩｇＶＨ区基因的引物，从分泌鼠抗ｈＴＮＦ－α单克隆抗体的Ｅ６杂交瘤细胞株中，分别扩增和克隆了自５′非翻译区至Ｃγ１近３′端的重链基因片段和重链可变区基因片段，并测定了其核苷酸序列。计算机分析表明，系重排的鼠Ｉｇ重链基因。
Using a pair of anchor PCR primers and a pair of primers for the murine IgVH gene, the E6 hybridoma cell strains secreting mouse anti-hTNF-α monoclonal antibody were amplified and cloned by PCR from 5 ’Untranslated region to the 3’ end of Cγ1 and the heavy chain variable region gene fragment, and the nucleotide sequence was determined. Computer analysis showed that the rearranged mouse Ig heavy chain gene.