论文部分内容阅读
目的:应用柯萨奇病毒B3(CVB3)腺病毒载体疫苗rAd/MDC-VP1初免,核酸疫苗pcDNA3/MDC-VP1加强免疫的策略免疫小鼠,观察其免疫效果。方法:BALB/c小鼠随机分为A～D 4组,分别肌肉注射PBS、rAd/MDC-VP1、pcD-NA3/MDC-VP1、rAd/MDC-VP1+pcDNA3/MDC-VP1,用ELISA和微量中和试验法分别检测CVB3 VP1特异性IgG和中和抗体滴度,CCK-8法检测脾淋巴细胞增殖活性和特异性CTL杀伤活性;用致死量的CVB3攻击小鼠后,检测小鼠血中病毒滴度并观察动物的存活情况。结果:D组CVB3 IgG、非特异性淋巴细胞增殖活性及特异性CTL杀伤活性明显高于其他各组(P<0.05);CVB3攻击后,D组小鼠血中病毒滴度较其他各组显著降低,生存率为41.67%,明显高于其他各组(P<0.05)。结论:rAd/MDC-VP1初免pcDNA3/MDC-VP1加强的免疫策略能显著提高小鼠细胞和体液免疫水平,提高致死量病毒攻击后的保护率。
OBJECTIVE: To immunize mice with recombinant adenovirus vector of coxsackievirus B3 (CVB3) vaccine rAd / MDC-VP1 and immunization with pcDNA3 / MDC-VP1 DNA vaccine to observe its immunogenicity. Methods: BALB / c mice were randomly divided into A ~ D 4 groups and intramuscular injection of PBS, rAd / MDC-VP1, pcD-NA3 / MDC-VP1, rAd / MDC-VP1 + pcDNA3 / CVB3 VP1-specific IgG and neutralizing antibody titers were detected by micro-neutralization assay, splenic lymphocyte proliferation activity and CTL-specific cytotoxic activity were detected by CCK-8 assay, and mice were challenged with lethal dose of CVB3 The virus titers were observed and the animals survived. Results: The CVB3 IgG, the nonspecific lymphocyte proliferation activity and the specific CTL killing activity in group D were significantly higher than those in other groups (P <0.05). After CVB3 challenge, the titer of blood in group D was significantly lower than that in other groups , The survival rate was 41.67%, significantly higher than the other groups (P <0.05). CONCLUSION: The boosted immunization strategy of rAd / MDC-VP1 naive pcDNA3 / MDC-VP1 can significantly increase the level of cellular and humoral immunity in mice and increase the protection rate after lethal virus challenge.