目的研究IL - 2基因修饰人肿瘤细胞生物学行为是否因辐射而发生变化。　方法分别从细胞形态结构、MHC分子表达、整合的基因IL - 2基因是否丢失、能否保持mRNA表达、能否分泌表达生物活性的IL - 2等方面进行了实验。结果未见整合的IL - 2基因丢失 ,能表达IL - 2mRNA且在一定时间内持续分泌具有生物活性的IL - 2 ;细胞膜表面MHC分子无明显改变 ;细胞形态结构无明显变化。结论经60 Co辐射修饰的肿瘤细胞致瘤性消失 ,但在一定时间内能分泌IL - 2 ,这为开展基因工程“瘤苗”的临床应用提供了依据。 Objective To investigate whether the biological behavior of human tumor cells modified by IL - 2 gene is changed by radiation. Methods The experiments were carried out in terms of cell morphology and structure, expression of MHC molecules, whether the integrated gene IL - 2 gene was lost, whether mRNA expression could be maintained, and secretion of IL - 2 expressing biological activity. Results No IL - 2 gene was found to be lost, IL - 2 mRNA could be expressed and IL - 2 with biological activity continued to be secreted within a certain period of time. There was no obvious change of MHC on the surface of cell membrane. Conclusion The tumorigenicity of tumor cells modified by 60Co radiation disappears, but can secrete IL - 2 within a certain period of time, which provides a basis for the clinical application of genetically engineered “tumor vaccine”.