【摘 要】
：
A new method for the determination of antithrombotic activity of egg white protein hydrolysate (EWPH) was developed using a microplate reader. Reaction was carr
【机 构】
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School of Food Science & Technology
论文部分内容阅读
A new method for the determination of antithrombotic activity of egg white protein hydrolysate (EWPH) was developed using a microplate reader. Reaction was carried out at 37 ℃ and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity (R2 = 0.9971), when the inhibition rate was in the range of 10-90%. The lower limit of detection (LLD, at 99.7% probability) and the biological limit of detection (BLD, at 99.7% probability) of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation (R.S.D.) was 1.08%. The standard deviation of the method was ±0.027 AT-U.
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