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目的探讨凋亡抑制蛋白Survivin、促细胞凋亡蛋白Fas在正常宫颈、宫颈上皮内瘤样变(CIN)和宫颈癌中的表达及其临床意义。方法应用免疫组化S-P法,检测Survivin、Fas蛋白在9例正常宫颈、15例CIN、34例宫颈鳞癌组织中的表达。所有标本均取自2000～2002年间在中国医科大学附属第一医院及辽宁省肿瘤医院就医的患者。结果Survivin、Fas在正常宫颈组织中的阳性表达率分别为0、88.89%;在CIN中的阳性表达率分别为40.00%、53.33%;在宫颈鳞癌中阳性表达率分别为67.67%、44.12%,二者在宫颈不同组织的表达差异有显著性(P<0.05)。Survivin表达与宫颈鳞癌临床分期及病理分级成正相关(P<0.05),Fas表达仅与病理分级成正相关(P<0.05)。Survivin表达随宫颈疾病发展有明显增加趋势,Fas表达有明显降低趋势,二者的阳性表达呈负相关。结论Survivin表达引起的细胞凋亡抑制,在宫颈癌的发生发展中起一定作用。Survivin基因的表达与宫颈癌组织中Fas蛋白的表达呈负相关。
Objective To investigate the expression and clinical significance of Survivin, a proapoptosis protein Fas in normal cervical and cervical intraepithelial neoplasia (CIN) and cervical cancer. Methods Immunohistochemical S-P method was used to detect the expression of Survivin and Fas protein in 9 cases of normal cervix, 15 cases of CIN and 34 cases of cervical squamous cell carcinoma. All samples were taken from 2000 to 2002 in the First Affiliated Hospital of China Medical University and Liaoning Provincial Cancer Hospital for patients. Results The positive rates of Survivin and Fas in normal cervical tissues were 0 and 88.89%, respectively. The positive rates in CIN were 40.00% and 53.33% respectively. The positive rates of Survivin and Fas in cervical squamous cell carcinoma were 67.67% and 44.12% There was a significant difference between the two tissues in different tissues of cervix (P <0.05). Survivin expression was positively correlated with clinical stage and pathological grade of cervical squamous cell carcinoma (P <0.05). Fas expression was only positively correlated with pathological grade (P <0.05). The expression of Survivin increased significantly with the development of cervical diseases, while the expression of Fas decreased significantly. The positive expression of Survivin was negatively correlated with the expression of Survivin. Conclusion The inhibition of apoptosis induced by Survivin may play a role in the occurrence and development of cervical cancer. Survivin gene expression and cervical cancer tissue protein expression was negatively correlated.