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在入贡湖河道亲水河采集上覆水、伊乐藻及底泥柱芯样品进行室内实验,探究伊乐藻与固定化高效脱氮微生物协同作用下脱除铵态氮的机制,以及对污染水体的生态修复效果.运用~(15)N同位素配对技术及高通量测序技术,研究了4个不同处理组中NH_4~+的转化机制,其中,处理组A:裸泥组,处理组B:固定化高效脱氮微生物,处理组C:伊乐藻,处理组D:固定化高效脱氮微生物+伊乐藻.结果表明,氮素脱除主要有底泥存储、伊乐藻吸收及微生物过程(反硝化和厌氧氨氧化)这3种途径.在添加沉水植物伊乐藻的处理组C和D中,伊乐藻对~(15)NH_4~+吸收率分别为25.44%和19.79%.不同处理组中底泥对~(15)NH_4~+存储率分别为7.94%、5.52%、6.47%和4.86%,微生物过程以气体形式释放的~(15)NH_4~+分别为16.06%、28.86%、16.93%和33.09%.反硝化和厌氧氨氧化是产生含氮素气体的主要过程,对于处理组D,脱氮微生物丰度和多样性均得到不同程度的提升.4个不同处理组对~(15)NH_4~+的总去除率分别为24%、34.38%、48.84%和57.74%,伊乐藻与高效脱氮微生物联用技术(EINCB)的应用,可以提高水体氮素的脱除速率,促进污染水体的净化.
The experiment was conducted on the samples of overlying water, Elodea alba and sediment core samples collected from the hydrophilic river in the Gonggong Lake, to explore the mechanism of the removal of ammonium nitrogen by synergistic interaction between Elodea alba and immobilized high-efficiency denitrifying microorganisms, The mechanisms of ecological restoration of water bodies were studied. The mechanism of NH 4 + conversion in four different treatment groups was studied using ~ (15) N isotope pairing technique and high-throughput sequencing technology. Among them, treatment group A: naked mud group, treatment group B : Immobilized high efficiency denitrifying microorganism, treatment group C: Elodea nuttallii, treatment group D: immobilized highly efficient denitrifying microorganism + Elodea nuttallii. The results showed that nitrogen removal mainly includes sediment storage, Elodea nuttallii absorption and microorganism (Denitrification and Anaerobic Ammonia Oxidation) .In the treatments C and D with the addition of submerged plant Elodea, the absorption rates of ~ (15) NH 4 + by Elodea nuttallii were 25.44% and 19.79, respectively %. The storage rates of ~ (15) NH_4 ~ + in sediments of different treatment groups were 7.94%, 5.52%, 6.47% and 4.86% respectively, and that of 15 NH4 + released by gas in microbial process was 16.06% , 28.86%, 16.93% and 33.09%, respectively. Denitrification and anaerobic ammonium oxidation were the main processes to produce nitrogen-containing gas. For treatment group D, the abundance of denitrifying microorganisms and The total removal rates of ~ (15) NH 4 + in the four different treatment groups were 24%, 34.38%, 48.84% and 57.74%, respectively. Technology (EINCB) application, can improve the removal rate of nitrogen in water body, and promote the purification of polluted water.