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目的探讨白细胞介素-12基因4个位点的单核苷酸多态性与宁夏回、汉族哮喘儿童与正常儿童间的相关性。方法应用PCR-RFLP技术对回、汉族哮喘患儿及回、汉族正常儿童各60例外周血样本进行分析,采用χ2检验对白细胞介素-12基因4个位点的基因型及基因频率的分布进行分析。结果白细胞介素-12基因4个位点1188(rs3212227)、rs14050306、rs15677380、rs6887695的基因与基因型频率的分布在宁夏回汉族哮喘儿童、回族哮喘及正常儿童和回汉族正常儿童之间无显著差异(P>0.05)。结论白细胞介素-12基因的4个位点与宁夏回族、汉族儿童哮喘相关性无显著差异。
Objective To investigate the association of single nucleotide polymorphisms at the four loci of interleukin-12 with Ningxia Hui and Han children with asthma and normal children. Methods The PCR-RFLP technique was used to analyze the peripheral blood samples from 60 children with asthma in the Han and Hui nationalities and in the normal Han and Han nationality. The genotype distribution and gene frequency of 4 IL-12 genes were analyzed byχ2 test Analyze. Results There was no significant difference in frequencies of genotypes and genotypes between 1188 (rs3212227), rs14050306, rs15677380 and rs6887695 at 4 loci in Ningxia Hui nationality asthma children, asthmatic children with asthma, normal children and normal Han children Difference (P> 0.05). Conclusion There is no significant difference between the four loci of IL-12 gene and asthma in Han and Hui children in Ningxia.