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目的构建一种基于分子信标与核酸适体的荧光传感器,用于三磷酸腺苷分子的选择性检测。方法将三磷酸腺苷的核酸适体序列与分子信标结合形成新的荧光探针,利用腺苷和分子信标互补DNA的竞争,引发分子信标的构型转换,从而产生可检测的荧光响应信号实现检测腺苷的目的。结果荧光响应信号随着腺苷浓度的增加而减弱。在3.70×10~(-6)~5.56×10~(-4)mol/L的浓度范围内,腺苷浓度的对数和响应信号成线性关系,相关系数为0.9974。加标回收率为92.0%~110.0%,相对标准偏差为0.68%~1.85%(n=3)。结论该方法耗时较少、步骤简单,可满足当前生化分析快速而精确的分析要求。
Objective To construct a fluorescence sensor based on molecular beacon and aptamer for the selective detection of adenosine triphosphate molecules. Methods The aptamer sequence of aptamer was combined with the molecular beacon to form a new fluorescent probe. By using the competition of complementary DNA between adenosine and molecular beacon, the configuration conversion of molecular beacon was initiated and a detectable fluorescent response signal was generated to detect The purpose of adenosine. Results The fluorescence response signal decreased with the increase of adenosine concentration. The logarithm of adenosine concentration was linear with the response signal in the concentration range of 3.70 × 10 -6 -6 5.5 × 10 -4 mol / L with a correlation coefficient of 0.9974. The spiked recoveries ranged from 92.0% to 110.0% with relative standard deviations of 0.68% to 1.85% (n = 3). Conclusion This method is less time-consuming and has simple steps to meet the fast and accurate analysis requirements of current biochemical analysis.