微管相关蛋白在植物生长发育过程中发挥重要作用。利用反转录聚合酶链式反应(RT-PCR)和快速扩增c DNA末端(RACE)技术对非洲菊(Gerbera hybrida)体内微管相关蛋白GMAP65-1基因进行了克隆,获得的基因全长1 883 bp,包含1 740 bp的完整开放阅读框(ORF)。表达模式研究表明,该基因在非洲菊幼嫩的根、叶及花中均有较高的表达,且受到赤霉素(GA)诱导显著上调。构建GMAP65-1超表达载体,经异源转化拟南芥(Arabidopsis thaliana)后筛选获得纯合株系,对纯合株系进行表型观察。结果表明,GMAP65-1超表达植株的叶片及花瓣面积增大,暗示该基因参与叶片及花瓣的形态建成。研究结果为花卉分子育种提供了理论依据及基因资源。 Microtubule-associated proteins play an important role in plant growth and development. The gene of microtubule-associated protein GMAP65-1 in Gerbera hybrida was cloned by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of c DNA terminus (RACE) 1 883 bp, contains a complete open reading frame (ORF) of 1 740 bp. The study of expression pattern showed that the gene was highly expressed in the young root, leaf and flower of Gerbera jamesonii and was significantly up-regulated by gibberellin (GA). The GMAP65-1 overexpression vector was constructed and transformed into Arabidopsis thaliana by heterologous transformation. The homozygous lines were screened and the phenotypes of the homozygous lines were observed. The results showed that the area of ​​leaves and petals of GMAP65-1 overexpression plants increased, suggesting that the gene involved in the formation of leaves and petals. The results provide a theoretical basis and gene resources for flower molecular breeding.