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目的:从基因水平进一步探讨四逆散调控实验性溃疡性结肠炎大鼠IL-4/STAT6信号通路的作用机制。方法:将实验动物分为6组,采用免疫法造模,以大鼠IL-4、SOCS1、STAT6的基因表达作为观察与检测指标。结果:四逆散组实验大鼠IL-4、SOCS1表达显著增加(P<0.05),而STAT6显著降低(P<0.05);三个拆方组中,柴芍配伍对IL-4、SOCS1 mRNA表达的影响与四逆散无显著性差异(P>0.05),但STAT6 mRNA的表达显著高于正常组(P>0.05);与柴枳甘组、芍枳甘组比较,柴芍组IL-4、SOCS1 mRNA表达明显升高(P>0.05),STAT6 mRNA的表达显著降低(P>0.05)。结论:四逆散能够通过刺激IL-4、SOCS1 mRNA的表达,抑制STAT6mRNA的表达来调节IL-4/STAT6通路,干预实验性UC。方中柴芍配伍作用明显,主要体现在对IL-4、SOCS1mRNA表达的影响上;柴、芍分别配伍柴枳后,对TL-4/STAT6通路的调节作用明显降低。
OBJECTIVE: To further explore the mechanism of IL-4 / STAT6 signaling pathway in SNS rats induced by ulcerative colitis at the gene level. Methods: The experimental animals were divided into 6 groups and used for immunological modeling. The gene expressions of IL-4, SOCS1 and STAT6 in rats were used as observation and detection indexes. Results: The expression of IL-4 and SOCS1 in Siniosan group were significantly increased (P <0.05), and STAT6 was significantly decreased (P <0.05). Among the three groups, (P> 0.05), but the expression of STAT6 mRNA was significantly higher than that of the normal group (P> 0.05). Compared with Chai Zhi Gan group and Shao Zhi Huang Gan group, the expression of IL- 4, SOCS1 mRNA expression was significantly increased (P> 0.05), STAT6 mRNA expression was significantly reduced (P> 0.05). Conclusion: Sini Decoction can regulate IL-4 / STAT6 pathway by interfering with the expression of IL-4 and SOCS1 mRNA and the expression of STAT6 mRNA in experimental UC. The effect of compatibility of Radix Paeoniae Alba is obvious, which is mainly reflected in the effect on the expression of IL-4 and SOCS1 mRNA. After the compatibility of Chai and Chai respectively, the regulation of TL-4 / STAT6 pathway is obviously reduced.