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目的:评价中药杠板归体内抗流感病毒的药效。方法:120只BABL/c小鼠适应性饲养7天后随机分成6组,每组20只,分别为正常组、病毒对照组、利巴韦林组、杠板归高剂量组、杠板归中剂量组、杠板归低剂量组。乙醚麻醉小鼠,以10LD_(50)流感病毒40μL滴鼻感染小鼠,1h后开始给药,正常组和病毒对照组灌胃生理盐水0.1mL/d,利巴韦林组灌胃利巴韦林100mg/(kg·d),杠板归高、中、低各剂量组分别灌胃杠板归水煎液20g/(kg·d)、10g/(kg·d)、5g/(kg·d)。连续给药7天,每天记录体质量变化以及感染小鼠死亡情况。第8天,眼球取血,检测血清中免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、免疫球蛋白G亚型1b(IgG1b)、免疫球蛋白G亚型2a(IgG2a)、白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)水平;摘取肺组织,称肺重,进行病理检测。结果:与正常组比较,病毒对照组小鼠肺指数、IL-1、TNF-α、IgA、IgG、IgG1b、IgG2a水平显著升高,差异均有统计学意义(P<0.05,P<0.01)。与病毒对照组比较,利巴韦林组和杠板归中剂量组小鼠肺指数显著降低;利巴韦林组、杠板归中剂量组、杠板归低剂量组小鼠炎症因子IL-1和TNF-α水平显著降低;利巴韦林组和杠板归中剂量组小鼠IgA、IgG、IgG1b、IgG2a水平显著升高,杠板归低剂量组小鼠IgA水平显著升高;差异均有统计学意义(P<0.05)。与利巴韦林组比较,杠板归中剂量组小鼠肺指数、炎症因子IL-1、IgA水平较高,TNF-α水平较低;杠板归低剂量组小鼠肺指数、炎症因子IL-1水平较高,IgG1b水平较低,差异均有统计学意义(P<0.05)。结论:有效剂量的中药杠板归能够提高流感病毒感染鼠的抗体水平,降低炎症因子含量以及肺指数,减轻肺部炎症,表现出较好的抗病毒作用。
OBJECTIVE: To evaluate the efficacy of anti-influenza virus in Chinese herbal medicine. Methods: One hundred and twenty BABL / c mice were randomly divided into 6 groups (n = 20) after 7 days of adaptive feeding. The mice in control group, ribavirin group, Dose group, low-dose plate group. Mice were anesthetized with diethyl ether, and mice were instilled nasally by injecting 40μL of 10LD_ (50) influenza virus into the nasal mucosa. After 1h, the mice were dosed with normal saline and normal saline. Rats in the ribavirin group were orally administered with ribavirin Lin 100mg / (kg · d), the bungee back to the high, middle and low doses of the respective groups were administered by the decoction of the pengban decoction 20g / (kg · d), 10g / (kg · d), 5g / (kg · d) d). Continuous administration of 7 days, daily record of body weight changes and the death of infected mice. On the 8th day, blood was collected from the eye to detect serum IgA, IgG, IgG1b, IgG2a, (IL-1) and tumor necrosis factor-α (TNF-α) were detected. Lung tissues were harvested and lung weights were taken for pathological examination. Results: Compared with the normal group, the levels of IL-1, TNF-α, IgA, IgG, IgG1b and IgG2a in virus control group were significantly increased (P <0.05, P <0.01) . Compared with the virus control group, the lung index in the ribavirin group and the midgut dose group was significantly lower; the ribavirin group, Baogu Guizhong dose group, the low dose Baogan group mice inflammatory cytokines IL- 1 and TNF-α levels were significantly decreased; the levels of IgA, IgG, IgG1b and IgG2a in ribavirin group and middle dose of LGP group were significantly increased, and the level of IgA in low-dose LGP group was significantly increased All were statistically significant (P <0.05). Compared with the ribavirin group, the lung index, inflammatory cytokines IL-1 and IgA levels were higher and the level of TNF-α was lower in the mice treated with low dose Baogan. The lung index, inflammatory factor The level of IL-1 was higher and the level of IgG1b was lower, the differences were statistically significant (P <0.05). Conclusion: The effective dose of Chinese herbal medicine can improve the level of antibody in mice infected with influenza virus, reduce the content of inflammatory cytokines and lung index, relieve the inflammation of the lung and show a good anti-virus effect.