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目的 :检测人单核细胞 (monocyte)和 CD34+ 干细胞来源的树突状细胞 (DC)—— Mo DC和 CD34DC,在分化发育不同阶段表达 TRAIL及 TRAIL受体 (TRAIL - Rs)的情况和外源性因子 L PS或 IFN-β刺激未成熟 DC后 TRAIL - Rs的表达变化。 方法 :采用 RT- PCR和 FACS两种方法检测人 DC表达 TRAIL和 TRAIL - Rs的水平。 结果 :人 CD34+ 干细胞在向CD34DC分化过程中 ,表达 TRAIL和 TRAIL - Rs的先后顺序为 DCR1和 DCR2 (+0 d) ,DR4和 DR5 (+5 d) ,TRAIL (+8d)。成熟 DC表达中度水平的 TRAIL、DR4、DR5、DCR2 ,不表达 DCR1。除了 DCR1外 ,Mo DC和未经诱导的单核细胞表达TRAIL及其 4个膜受体的水平基本一致 ,DCR1在诱导过程中逐渐降低。未成熟的 Mo DC在 L PS的刺激下 ,表达 DR5和 DR4的水平增高 ,在 IFN -β刺激下表达 DR5的水平增高。结论 :TRAIL和 TRAIL - Rs可能参与 DC分化或其功能的发挥。L PS或IFN-β能够增加 DC对 TRAIL诱导凋亡的敏感性 ,表明它们参与机体的免疫调控。
AIM: To detect the expression of TRAIL and TRAIL receptors (TRAIL - Rs) in dendritic cells (DCs) derived from human monocytes and CD34 + stem cells, The change of TRAIL - Rs expression in immature DC stimulated by factor L PS or IFN-β. Methods: The expressions of TRAIL and TRAIL - Rs in human DCs were detected by RT - PCR and FACS. Results: The order of expression of TRAIL and TRAIL - Rs in human CD34 + stem cells was DCR1 and DCR2 (+0 d), DR4 and DR5 (+5 d) and TRAIL (+8 d) during differentiation into CD34 DC. Mature DCs express moderate levels of TRAIL, DR4, DR5, DCR2, but do not express DCR1. Except for DCR1, the level of TRAIL and its four membrane receptors expressed by Mo DC and uninucleated monocytes were basically the same, while DCR1 gradually decreased during induction. Immature Mo DC stimulated by L PS, the level of expression of DR5 and DR4 increased, the level of expression of DR5 increased under the stimulation of IFN-β. Conclusion: TRAIL and TRAIL - Rs may play roles in DC differentiation or its function. L PS or IFN-β can increase the sensitivity of DC to TRAIL-induced apoptosis, indicating that they are involved in the body’s immune regulation.