目的:比较half-ICSI与备早补救ICSI 2种授精方式在体外受精周期中的应用价值。方法:选取可疑受精失败的不孕患者,随机分为half-ICSI组(A组,half-ICSI授精,138个周期)和行备早补救ICSI组(B组,行备早补救ICSI授精,112个周期)。分析受精率、胚胎质量、种植率和妊娠率。结果:A组与B组的正常受精率分别为65.37%和70.78%,多精受精率分别为1.59%和3.08%,优质胚胎率分别为66.76%和73.75%,胚胎种植率分别为26.64%和35.59%,组间均有统计学差异(P<0.05);妊娠率分别为46.09%和57.84%,组间无统计学差异(P>0.05)。其中在IVF受精成功周期中,A组的优质胚胎率、胚胎种植率和临床妊娠率均明显低于B组(P<0.05);而在IVF受精失败周期中,ICSI的优质胚胎率、胚胎种植率和临床妊娠率A、B组间均无差异(P>0.05),但A组的受精率显著低于B组(P<0.01)。结论:备早补救ICSI授精方式优于half-ICSI授精方式,但有增加多精受精的风险。 Objective: To compare the application value of half-ICSI and ICSI two kinds of insemination in IVF cycle. Methods: Infertile patients with suspicious fertilization failure were randomly divided into half-ICSI group (group A, half-ICSI insemination, 138 cycles) and ICSI group (group B, ICSI Cycle). Analysis of fertilization rate, embryo quality, implantation rate and pregnancy rate. Results: The normal fertilization rates of group A and group B were 65.37% and 70.78%, respectively. The fertilization rates of fertilization were 1.59% and 3.08%, the rates of high quality embryos were 66.76% and 73.75% respectively, and the implantation rates of embryos were 26.64% and 35.59% respectively. The pregnancy rates were 46.09% and 57.84% respectively, with no significant difference between the two groups (P> 0.05). Among the successful cycles of IVF fertilization, the quality embryo rate, embryo implantation rate and clinical pregnancy rate in group A were significantly lower than those in group B (P <0.05). However, in IVF fertilization failure cycles, There was no difference between the two groups (P> 0.05), but the fertilization rate of group A was significantly lower than that of group B (P <0.01). CONCLUSIONS: Early recovery ICSI insemination is superior to half-ICSI insemination but increases the risk of multiple fertilization.