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选取产前30d荷斯坦牛120头(其中临产头胎牛和经产牛各60头),分别于产前21d、14d、7d、产后0h、6h、12h、24h、5d、10d、15d、21d、30d采血,测定血液酸碱度(pH)、血红蛋白(HB)、红细胞压积(HCT)、二氧化碳分压(P_(CO_2))、氧分压(P_(O_2))、剩余碱(BE)、钾(K)、钠(Na)等血气指标,确定围产期荷斯坦牛血气变化规律,初步建立北京地区荷斯坦牛血气指标的正常值参考范围。
A total of 120 Holstein cattle (including the first-born and the second-born 60) were collected 30 days prenatal and at 21d, 14d, 7d and 0h, 6h, 12h, 24h, 5d, 10d, 15d, Blood samples were collected 30 days later to measure blood pH, hemoglobin, HCT, P_ (CO_2), P_ (O_2), residual alkali (BE) and potassium (K), sodium (Na) and other blood gas indicators to determine perinatal Holstein cattle blood gas changes, the initial establishment of Beijing Holstein cattle blood gas index normal reference range.