论文部分内容阅读
目的探讨NS-398对肝细胞癌MVD值、VEGF、MMP-9表达的影响。方法采用RT-PCR和流式细胞术检测肝癌SMMC-7721细胞VEGF、MMP-9表达。采用免疫组化法检测裸鼠移植瘤MVD,ELISA法测定血清中PGE2水平。结果在体外,NS-398可以呈剂量和时间依赖性显著抑制SMMC-7721细胞的VEGF、MMP-9 mRNA和蛋白表达;在体内,可显著降低裸鼠移植瘤MVD、VEGF、MMP-9基因和蛋白表达(P<0.001),并能降低裸鼠血清中PGE2水平(P<0.001)。结论COX-2与肝细胞癌血管生成密切相关,NS-398可通过下调VEGF和MMP-9的表达而抑制肝细胞癌血管生成。
Objective To investigate the effect of NS-398 on the expression of MVD, VEGF and MMP-9 in hepatocellular carcinoma. Methods RT-PCR and flow cytometry were used to detect the expression of VEGF and MMP-9 in hepatoma SMMC-7721 cells. MVD was detected by immunohistochemistry in nude mice and PGE2 level in serum by ELISA. Results In vitro, NS-398 significantly inhibited the expression of VEGF and MMP-9 mRNA and protein in SMMC-7721 cells in a dose-and time-dependent manner. In vivo, NS-398 significantly reduced the expression of MVD, VEGF and MMP- (P <0.001), and decreased the level of PGE2 in the serum of nude mice (P <0.001). Conclusions COX-2 is closely related to angiogenesis of hepatocellular carcinoma. NS-398 can inhibit angiogenesis of hepatocellular carcinoma by down-regulating the expression of VEGF and MMP-9.