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目的:了解扬州市8类食品中Lm污染状况、流行特征及耐药状况,为制定HACCP提供依据,以有效控制Lm的生物性危害。方法:从市场及宾馆饭店采集957份熟食、生肉制品、水产品等8类食品检测Lm,检出菌株以生化及PCR方法鉴定并做药物敏感试验。结果:共检出Lm 43株,检出率为4 49%。其中熟肉制品检出率为9. 62%,生肉制品为3. 83%,蔬菜、牛奶、鲜鸡蛋及冰淇淋中未检出。17种抗生素中有9种抗生素出现了不同程度的耐药。结论:本市8种食品中存在一定程度Lm污染,以熟食污染率最高。生肉制品为食品链中Lm的主要来源,熟食是实施HACCP控制Lm的关键环节。Lm耐药现象在一定程度上存在。加强食品卫生管理尤其是熟肉制品的管理,控制动物饲料亚治疗抗生素的使用并严格遵守休药期对防止耐药菌株、控制食源性疾病很有必要。
Objective: To understand the status of Lm contamination, epidemic characteristics and drug resistance in 8 kinds of food in Yangzhou, and to provide the basis for the development of HACCP to effectively control the biological damage of Lm. Methods: A total of 957 samples of cooked foods, raw meat products and aquatic products were collected from the market and guesthouses and restaurants. The strains were identified by biochemical and PCR methods and tested for drug susceptibility. Results: A total of 43 Lm strains were detected with a detection rate of 494%. Among them, the detection rate of cooked meat products was 9.22%, that of raw meat products was 3.83%, and not detected in vegetables, milk, fresh eggs and ice cream. Nineteen kinds of antibiotics in 17 kinds of antibiotics appeared different degrees of resistance. Conclusion: There is a certain degree of Lm pollution in 8 kinds of food in this city, with the highest rate of cooked food contamination. Raw meat products are the main source of Lm in the food chain, and cooked food is a key link in the implementation of HACCP control Lm. Lm resistance exists to some extent. Strengthening the management of food hygiene, especially the management of cooked meat products, it is necessary to control the use of sub-therapeutic antibiotics in animal feed and strictly observe the withdrawal period to prevent drug-resistant strains and control food-borne diseases.