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目的:在HPLC指纹图谱和降低急性血瘀大鼠全血黏度药效的基础上进行谱效分析,以此确定虎杖降低急性血瘀大鼠全血黏度药效的“药效成分组”。方法:采用新型的多元统计分析方法——偏最小二乘回归法,对虎杖HPLC指纹图谱数据和降低急性血瘀大鼠全血黏度药效数据之间的内在联系进行谱效分析,得到指纹图谱中代表化学成分的各个峰的峰面积对药效的回归系数。结果:指纹图谱上的X1、X2、X7、X13、X17、X21这6个成分组成的“功效成分组”对虎杖降低全血黏度药效的贡献最大。结论:中医用药基于正确的辨证论治,是根据中药的功效对患者进行治疗,因此,反映中药功效的“功效成分组”是评价中药质量的关键。
OBJECTIVE: To determine the effect of Polygonum cuspidatum on reducing whole blood viscosity of acute blood stasis rats by HPLC fingerprinting and reducing the whole blood viscosity pharmacodynamics of acute blood stasis rats. . Methods: A new multivariate statistical analysis method, partial least-squares regression was used to analyze the relationship between the HPLC fingerprint of Polygonum cuspidatum and the pharmacodynamic data of whole blood viscosity in acute blood stasis rats to obtain the fingerprint In the chemical composition of the peak area of each peak of the regression coefficient of efficacy. Results: The six components of X1, X2, X7, X13, X17 and X21 on the fingerprints were the most effective contributors to reducing the whole blood viscosity of Polygonum cuspidatum. Conclusion: Based on correct diagnosis and treatment based on correct syndrome differentiation, traditional Chinese medicine treatment is based on the efficacy of traditional Chinese medicine. Therefore, the “effective ingredient group” reflecting the efficacy of traditional Chinese medicine is the key to evaluate the quality of traditional Chinese medicine.