论文部分内容阅读
目的探讨TNF-α、IL-6基因对高血糖“代谢记忆”的调控作用。方法根据正常对照(NC)组、糖尿病(DM)组、代谢记忆组对90只雄性健康SD大鼠进行造模,各30只。RT-PCR测定mRNA表达;TNF-α、IL-6的RT-PCR产物电泳条带积分光密度值(ROD);TNF-α、IL-6指标在血清中的表达情况。结果与NC组比较,代谢记忆组TNF-α、IL-6的RT-PCR产物ROD呈降低趋势,DM组呈升高趋势(P<0.05);与NC组比较,代谢记忆组血清中TNF-α、IL-6水平呈降低趋势(P<0.05),DM组呈升高趋势(P<0.05)。结论 NC组TNF-α和IL-6的mRNA低于DM组,代谢记忆组(高血糖16~32mmol/L 12周在先,正常血糖5~8mmol/L 12周在后)的相应mRNA低于NC组。同上的规律在血清TNF-α和IL-6水平再次出现。这是临床糖尿病代谢记忆的分子生物学基础在TNF-α和IL-6的表现。
Objective To investigate the regulatory effect of TNF-α and IL-6 on hyperglycemia and metabolic memory. Methods Ninety male Sprague-Dawley rats were randomly divided into normal control (NC), diabetic (DM) and metabolic memory groups (n = 30). The mRNA expression of TNF-α and IL-6 were determined by RT-PCR. The integral optical density (ROD) values of RT-PCR products were determined by RT-PCR. Results Compared with NC group, ROD of RT-PCR products of TNF-α and IL-6 in metabolic memory group tended to decrease, while that in DM group increased (P <0.05). Compared with NC group, TNF- α, IL-6 levels decreased (P <0.05), DM group increased (P <0.05). Conclusions The mRNA expression of TNF-α and IL-6 in NC group is lower than that in DM group. The mRNA expression in metabolic memory group (hyperglycemia of 16 ~ 32mmol / L for 12 weeks, normal glucose 5 ~ 8mmol / L for 12 weeks) . The same pattern appears again at the serum levels of TNF-α and IL-6. This is the molecular basis of clinical metabolic memory of diabetes in the expression of TNF-alpha and IL-6.