The traditional technique for asexual propagation of Siraitia grosvenori in vitro is adopted widely by pressing the vine, which has a high risk of carrying viral diseases and limits the production of promoted cultivars. So this paper reported in vitro regeneration of S. grosvenori by testing for shoot induction from various explant sources such as leaf, petiole and stem. Several phytohormone combinations of TDZ, BA and IAA were examined for shoot regeneration and NAA or IBA for rooting. The highest shoot induction rate (100% of regeneration frequency and 15.3 shoots per explant) in leaf was obtained by incubation on MS medium supplemented with 0.5 mg · L-1 TDZ, 2.0 mg · L-1 BA and 0.5 mg · L-1 IAA; unlike shoot regeneration in leaves, the most efficient bud inductions in petiole and stem explants were initiated on MS medium containing 0.2 mg · L-1 TDZ, 2.0 mg · L-1 BA and 0.5 mg · L-1 IAA, in addition, adventitious buds in petiole and stem explants needed to be transformed to MS medium for development; optimal root was obtained when shoots were cultured on 1/2MS medum supplemented with 0.5 mg · L-1 NAA or 0.5 mg · L-1 IBA. The traditional technique for asexual propagation of Siraitia grosvenori in vitro is favored widely by pressing the vine, which has a high risk of carrying viral diseases and limits the production of promoted cultivars. So this paper reported in vitro regeneration of S. grosvenori by testing for shoot induction from various explant sources such as leaf, petiole and stem. Several phytohormone combinations of TDZ, BA and IAA were examined for shoot regeneration and NAA or IBA for rooting. The highest shoot induction rate (100% of regeneration frequency and 15.3 shoots per explant) in leaf was obtained by incubation on MS medium supplemented with 0.5 mg · L-1 TDZ, 2.0 mg · L -1 BA and 0.5 mg · L -1 IAA; unlike shoot regeneration in leaves, the most efficient bud inductions in petiole and stem explants were initiated on MS medium containing 0.2 mg · L-1 TDZ, 2.0 mg · L -1 BA and 0.5 mg · L -1 IAA, in addition, adventitious buds in petiole and stem explants needed to be transformed to MS medium for development; optimal root was obtained when shoots were cultured on 1 / 2MS medum supplemented with 0.5 mg · L-1 NAA or 0.5 mg · L-1 IBA.