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目的 探讨胶质瘤中血管内皮生长因子 (VEGF)受体KDR和FLT 1的基因表达水平及其与肿瘤病理的关系。方法 应用逆转录PCR(reversetranscriptionpolymerasechainreaction ,RT PCR)方法检测 4 3例胶质瘤和 5例正常脑组织标本中KDR和FLT 1的基因表达情况 ,结合凝胶分析仪半定量的检测肿瘤标本中特异性mR NA的含量 ,并与肿瘤的临床病理进行比较。结果 电泳结果 :FLT 1在 6 4 5bp左右可见一明亮的条带 ;而KDR在1 0 0 0bp以上 (1 2 4 1bp)可见一明亮的条带 ,其亮度均随肿瘤级别的升高而升高。FLT 1mRNA的相对含量Ⅰ～Ⅳ级依次为 :0 .6 4± 0 .0 8、0 .86± 0 .1 0、1 .2 3± 0 .0 6、1 .37± 0 .0 6 ;正常脑组织为 0 .34± 0 .0 4。KDRmRNA的相对含量Ⅰ～Ⅳ级依次为 :0 .70± 0 .0 8、0 .92± 0 .1 1、1 .6 2± 0 .36、2 .86± 0 .30 ,正常脑组织为 0 .4 0± 0 .0 7。VEGF两种受体mRNA的相对含量随肿瘤级别的增加而增加 ,有显著性差异 (P <0 .0 0 0 1 )。结论 两种受体之间的差异明显 ,总的来说 ,KDR要比FLT 1的mRNA含量高 ,而随着胶质瘤恶性程度的增加 ,这种差别明显增大 ,尤其在胶质母细胞瘤中 ,KDR的平均相对mRNA含量是FLT 1的 2倍多
Objective To investigate the gene expression of vascular endothelial growth factor (VEGF) receptors KDR and FLT 1 in gliomas and its relationship with tumor pathology. Methods The gene expressions of KDR and FLT 1 in 43 gliomas and 5 normal brain tissues were detected by reverse transcriptase polymerase chain reaction (RT PCR). The specificity of gel analyzer was tested by semiquantitative method mR NA content, and compared with the clinical pathology of the tumor. Results The results of electrophoresis showed a bright band around 644bp in FLT 1, while a bright band with a KDR above 120bp (122bp) showed a brightness increase with tumor grade high. The relative contents of FLT 1 mRNA in order of Ⅰ ~ Ⅳ were: 0. 64 ± 0. 0 8,0. 86 ± 0 .1 0,1 .2 3 ± 0 .0 6,1 .37 ± 0. 0 6; Normal brain tissue was 0.34 ± 0. 0 4. The relative contents of KDR mRNA from Ⅰ to Ⅳ were as follows: 0.70 ± 0.08,0.92 ± 0.11,1.62 ± 0.36,2.86 ± 0.30, normal brain tissue was 0 .4 0 ± 0 .0 7. The relative content of VEGF receptor mRNA with tumor grade increased, there was a significant difference (P <0.0001). Conclusions The difference between the two receptors is obvious. In general, KDR is higher than that of FLT 1 mRNA. However, with the increase of malignant degree of glioma, the difference is obviously increased, especially in glioblastoma In tumors, the mean relative mRNA level of KDR was more than twice that of FLT 1