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目的研究油松松针中的黄酮类成分,为松属植物的化学分类学研究提供科学依据。方法采用反复硅胶、聚酰胺、ODS、Sephadex LH-20柱色谱等方法进行分离纯化,根据理化性质和1H-NMR、13C-NMR、质谱等技术对分离得到的化合物进行结构鉴定。结果从油松松针中分离得到4个化合物,分别鉴定为山柰酚-3-O-(3″-O-反式-对-肉桂酰基)-(6″-O-反式-阿魏酰基)-β-D-吡喃葡萄糖苷[kaempferol 3-O-(3″-O-E-p-coumaroyl)-(6″-O-E-feruloyl)-β-D-glucopyranoside,1]、山柰酚-3-O-(3″,6″-二-反式-对-肉桂酰基)-β-D-吡喃葡萄糖苷[kaempferol3-O-(3″,6″-di-O-E-p-coumaroyl)-β-D-glucopyranoside,2]、山柰酚-3-O-(3″-反式-对-肉桂酰基)-β-D-吡喃葡萄糖苷[kaempferol3-O-(3″-O-E-p-coumaroyl)-β-D-glucopyranoside,3]、异鼠李素-3-O-β-D-吡喃葡萄糖苷[isorhamnetin3-O-β-D-glucopyranoside,4]。结论化合物1、3为首次从松属植物中分离得到,化合物2、4为首次从该种植物中分离得到。
Objective To study the flavonoids in pine needles of Pinus tabulaeformis and provide a scientific basis for the chemical taxonomy of Pinus. Methods The compounds were separated and purified by repeated silica gel, polyamide, ODS and Sephadex LH-20 column chromatography. The structures of the isolated compounds were identified by 1H-NMR, 13C-NMR and MS-MS. Results Four compounds were isolated from Pinus tabulaeformis and identified as kaempferol-3-O- (3 “-O-trans-p-cinnamoyl) β-D-glucopyranoside, kaempferol 3-O- (3 ”-OEp-coumaroyl) - (6“ -OE-feruloyl) - (3 ”, 6“ -di-O-p-coumaroyl) -β-D-glucopyranoside glucopyranoside, 2], kaempferol 3-O- (3 ”-OEp-coumaroyl) -β-D-glucopyranoside, D-glucopyranoside, 3], isorhamnetin 3-O-β-D-glucopyranoside (4). Conclusions Compounds 1 and 3 were isolated from Pinus for the first time and compounds 2 and 4 were isolated from this species for the first time.