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本文研究了大剂量IL-2短期冲击人胎脾单个核细胞(FSMC)后,其杀伤活性、增殖活性、表面抗原表达的时间动力学变化。结果表明:快速诱导人胎脾LAK细胞时,IL-2的最适剂量是5000UIL-2/1.0×10~7cell/ml。在第2、3d,快速诱导的人胎脾LAK细胞的杀伤活性及增殖活性均显著高于常规LAK(P<0.05,P<0.05)。且两者表型基本相同。快速诱导后不洗去IL-2的LAK细胞的杀伤、增殖活性显著、极显著地高于常规LAK(P<0.05,P<0.01),在后期,也不低于甚至高于常规LAK细胞。这些结果提示,快速诱导的人胎脾LAK细胞有可能用于临床,从而大大简化操作,减少污染机会;先用5000UIL-2/1.0×10~7cell/ml冲击1h,再按常规方法培养是一种独立且更优的方法。
This study investigated the time-dependent kinetics of killing activity, proliferative activity, and surface antigen expression of human fetal spleen mononuclear cells (FSMC) after high-dose IL-2 exposure. The results showed that the optimal dose of IL-2 was 5000 UIL-2/1.0×10~7cells/ml when LAK cells were rapidly induced. On the 2nd and 3rd day, the cytotoxic activity and proliferation activity of human fetal spleen LAK cells were significantly higher than those of conventional LAK (P<0.05, P<0.05). And the two are basically the same phenotype. The killing and proliferation activities of LAK cells that did not wash out IL-2 after rapid induction were significantly higher than conventional LAK (P<0.05, P<0.01), and were not lower or even higher than conventional LAK cells in the later period. These results suggest that rapidly induced human fetal spleen LAK cells may be used clinically, thus greatly simplifying the operation and reducing the chance of contamination; the first use of 5000UIL-2/1.0×10~7cell/ml for 1 hour, followed by routine culture An independent and better method.