论文部分内容阅读
目的探讨居室空气中主要挥发性化学物对小鼠肺、肝、肾、睾丸组织O6-DNA甲基转移酶(O6-methylguanine-DNA methyltransferase,MGMT)表达的影响。方法根据20户新装修居室空气中甲醛、苯、甲苯、二甲苯和氨的平均质量百分构成比采用化学纯品配制混合染毒液(0.9124g/ml)。将昆明种小鼠随机分为4组,低剂量组(7.5mg/m)3、中剂量组(15mg/m3)、高剂量组(30mg/m3),每组10只。在静式染毒柜中每天染毒1次,每次4h,共染毒9周。对照组不加混合物,在染毒柜中放置时间与染毒组相同。应用链霉亲和素生物-过氧化物酶(SABC)免疫组化法对小鼠肺、肝、睾丸、肾组织中MGMT进行检测。结果高、中、低剂量染毒组小鼠肝、肺、睾丸组织中MGMT表达降低,与对照组比较,差异有统计学意义(P<0.05,P<0.01)。肾组织中MGMT表达各组间差异无统计学意义(P>0.05)。结论居室空气中主要挥发性化学物对小鼠肝、肺、睾丸组织MGMT表达可能具有抑制作用。
Objective To investigate the effect of major volatile chemicals in the air of the bedroom on the expression of O6-methylguanine-DNA methyltransferase (MGMT) in lung, liver, kidney and testis in mice. Methods According to the average mass percentages of formaldehyde, benzene, toluene, xylene and ammonia in room air of 20 newly renovated houses, mixed poison solution (0.9124g / ml) was prepared by using chemical pure product. The Kunming mice were randomly divided into 4 groups: low dose group (7.5mg / m), middle dose group (15mg / m3) and high dose group (30mg / m3) In a static poisoning cabinet once a day, every 4h, a total of 9 weeks. The control group without mixture, in the exposure cabinet placed the same time with the exposure group. The MGMT in lung, liver, testis and kidney of mice was detected by streptavidin bioperoxidase (SABC) immunohistochemistry. Results The MGMT expression in liver, lung and testis of mice in high, medium and low dose groups was significantly lower than that in control group (P <0.05, P <0.01). The expression of MGMT in renal tissues showed no significant difference between groups (P> 0.05). Conclusion The main volatile chemicals in room air may inhibit the expression of MGMT in liver, lung and testis tissue of mice.