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三年中，研究了番茄组培苗的快繁技术，完成了从番茄外植体选择、分化培养、生根培养到试管苗驯化移栽入温室生产的一系列试验研究工作，为进一步在全日光温室实现工厂化育苗奠定技术上的基础。通过大量试验研究认为：适于快繁的番茄外植体为带腋芽的茎段和茎尖；适于分化的培养基为ＭＳ＋ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．０３ｍｇ／Ｌ＋ＧＡ０．０３ｍｇ／Ｌ；适于试管苗生根的培养基为ＭＳ＋ＮＡＡ０．０３ｍｇ／Ｌ；适于试管苗过渡移栽的基质为细砂加少量营养液。在最适培养激素配比条件下，外植体每继代一次的增殖系数为３．０～３．４，若以年增殖代数１０代计，则一个外植体的年增殖倍数为３．０１０～３．４１０，即５９０４９～２０６４３７倍。同时，试管苗移栽入温室后，仍正常地保持了优良番茄品种的种性，试管苗移栽成活率达到了８０％以上。
In the past three years, the rapid propagation technology of tomato tissue culture seedlings was studied. A series of experimental studies were carried out on tomato plant explants selection, differentiation and culture, rooting culture, domestication of test tube seedlings and transplanting into greenhouse. Greenhouse to achieve industrialized seedling laid the technical foundation. Through a large number of experimental studies, it is concluded that: the explants of tomato which are suitable for fast propagation are the stem segments and shoot tips with axillary buds; the medium suitable for differentiation is MS + BA 2.0 mg / L + NAA 0.03 mg / L + GA 0.03 mg / L; Rooting medium is MS + NAA0.03mg / L; suitable for transplanting of test tube seedling matrix is fine sand plus a small amount of nutrient solution. Under optimal conditions, the multiplication coefficient of explants was 3.0-3.4 per subculture, and the annual multiplication rate of one explant was 3 if the propagule was on the 10th generation. 010 ~ 3.410, namely 59049 ~ 206437 times. At the same time, the test tube seedlings transplanted into the greenhouse, still maintain the normal varieties of good seed, survival rate of transplanting test tube seedlings reached more than 80%.