我们采用小分子量的酸性蛋白质——绿豆胰蛋白酶抑制剂为配体,人工模拟了天然植物型(2Fe-2S)铁氧还蛋白(简称Fd)。模拟物Ⅰ的穆斯堡尔谱为两组四极分裂谱。其中一组(S峰)的谱参数与天然植物型Fd相似,另一组双峰(C峰)在植物型Fd中未发现。模拟物Ⅰ的磁性测量及ESR测量结果也与植物型Pd(氧化态)不同。 改进了模拟条件,增加了一试剂Na_2S·3H_2O,得到的结果为模拟物Ⅱ。其穆斯堡尔谱仅有一组双峰,谱参数与天然植物型Fd相近,I.S=0.24mm/s,(相对于α-Fe),Q.S=0.65mm/s。这说明其中的铁均为高自旋 We used a small molecular weight acidic protein - mung bean trypsin inhibitor as a ligand to simulate natural plant-type (2Fe-2S) ferredoxin (Fd). Mossbauer spectrum of simulant I is two sets of quadrupole splitting spectrum. The spectral parameters of one group (S peak) were similar to that of native plant type Fd, and the other group of double peaks (C peak) was not found in plant type Fd. The mock I and ESR measurements are also different from the plant-type Pd (oxidation state). The simulation conditions were improved, a reagent Na_2S · 3H_2O was added, and the result was simulant Ⅱ. The Mössbauer spectrum has only one set of bimodals, and the spectral parameters are similar to those of the native plant type Fs. I.S. = 0.24 mm / s (relative to α-Fe) and Q.S = 0.65 mm / s. This shows that the iron is high spin