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目的:探讨基质金属蛋白酶-1(MMP-1)基因1G/2G单核苷酸多态性(SNP)与福建地区胃癌人群生存及器官转移之间的可能关系。方法:对102例完整随访的胃癌患者采用外周血基因组DNA,运用PCR-变性高效液相色谱分析(DHPLC)方法进行MMP-1基因1G/2G多态性的基因型鉴定,比较基因型分布差别,以及与胃癌预后及随访过程中器官转移部位的潜在关系。统计学方法采用SPSS软件进行χ2检验、Kaplan-Meier生存分析和Log-rank等计算。结果:胃癌人群中MMP-1基因1G/2G多态性各基因型分布频率分别为1G/1G(15,14.7%),1G/2G(48,47.1%)和2G/2G(39,38.2%);与含1G基因型相比,2G/2G等位基因型携带者的生存率出现非显著性下降趋势,P=0.075;携带2G纯合等位基因型的胃癌患者随访中发生肝脏转移的机会明显加大,P=0.013。结论:MMP-1基因2G/2G基因型可能是影响福建地区胃癌患者生存的不良预后因子之一,携带2G纯合等位基因型可能是发生肝脏转移的危险因素。
Objective: To investigate the possible relationship between 1G / 2G single nucleotide polymorphisms of matrix metalloproteinase-1 (MMP-1) gene and the survival and metastasis of gastric cancer in Fujian. Methods: Genomic DNA of peripheral blood was collected from 102 complete gastric cancer patients. The genotypes of 1G / 2G polymorphism of MMP-1 gene were identified by PCR-denaturing high performance liquid chromatography (DHPLC) , And the potential relationship with the prognosis of gastric cancer and the site of organ metastasis during follow-up. Statistical methods using SPSS software χ2 test, Kaplan-Meier survival analysis and Log-rank calculation. Results: The genotype frequency of 1G / 2G polymorphism of MMP-1 gene in gastric cancer was 1G / 1G (15,14.7%), 1G / 2G (48,47.1%) and 2G / 2G ); Compared with the 1G genotype, there was an insignificant decrease in the survival rate of 2G / 2G allele carriers, P = 0.075; liver metastasis occurred in follow-up patients with 2G homozygous allele Opportunity increased significantly, P = 0.013. Conclusion: The genotypes of 2G / 2G of MMP-1 gene may be one of the adverse prognostic factors for the survival of patients with gastric cancer in Fujian Province. Carrying 2G homozygous allele may be the risk factor of liver metastasis.