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目的研究α-突触核蛋白(α-SYN)在大鼠不同脑区线粒体的表达及对线粒体呼吸链复合体Ⅰ活性的影响。方法用免疫印迹方法检测不同脑区线粒体中α-SYN表达量的变化并测定α-SYN向分离线粒体的转运;通过观察340nm处NADH吸收值的变化测定线粒体呼吸链复合体Ⅰ的活性。结果线粒体α-SYN在不同脑区的表达量不同。人重组α-SYN与分离线粒体进行孵育后,以剂量依赖的方式转运到线粒体内。将不同浓度人重组α-SYN与线粒体孵育后,线粒体复合体Ⅰ活性下降,并且呈明显的量-效关系。结论线粒体α-SYN在不同脑区的表达量不同。线粒体的α-SYN有可能来源于胞质α-SYN向线粒体的转运,并调节复合体Ⅰ的活性。
Objective To study the effects of α-synuclein (α-SYN) on the mitochondria and the activity of mitochondrial respiratory chain complex Ⅰ in different brain regions of rats. Methods The changes of mitochondrial α-SYN expression in different brain regions were detected by Western blotting, and the transport of α-SYN to isolated mitochondria was measured. The activity of mitochondrial respiratory chain complex Ⅰ was determined by observing the change of NADH absorption at 340nm. Results The expression of mitochondrial α-SYN was different in different brain regions. After human recombinant α-SYN was incubated with isolated mitochondria, the recombinant human α-SYN was translocated into mitochondria in a dose-dependent manner. After incubation of mitochondria with different concentrations of human recombinant a-SYN, the activity of mitochondrial complex I decreased, and showed a significant dose-response relationship. Conclusion The expression of mitochondrial α-SYN in different brain regions is different. Mitochondrial α-SYN may be derived from cytoplasmic α-SYN to mitochondrial transport, and regulate the activity of complex Ⅰ.