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目的制备法医学检验所用的确定人血的免疫胶体金层析试剂条。方法选取抗人血红蛋白单克隆细胞株,制备其小鼠腹水,从腹水中纯化出单克隆抗体。制备胶体金并用一纯化的单克隆抗体包被,制成免疫胶体金。取玻璃纤维以免疫胶体金浸泡,烘干。在一硝酸纤维素膜上两个不同位置分别点加另一抗人血红蛋白抗体和羊抗鼠IgG。搭建试剂条并检测其灵敏度和特异性。结果制成的免疫胶体金试剂条可对稀释至20万倍的人血红蛋白溶液显示阳性,对法医学检验常见8种动物的血溶液显示阴性。结论所制备抗人血红蛋白胶体金试剂条可以应用于法医学检验。
Objective To prepare human blood immunogold gold chromatography reagent strips used in forensic tests. Methods Monoclonal anti-human hemoglobin cell line was selected to prepare mouse ascites and monoclonal antibodies were purified from ascites. Prepared colloidal gold and coated with a purified monoclonal antibody, made of immune colloidal gold. Take glass fiber to immune colloidal gold soak, drying. Another anti-human hemoglobin antibody and goat anti-mouse IgG were spotted at two different locations on a nitrocellulose membrane. Set up a reagent strip and test its sensitivity and specificity. The results of the immune colloidal gold reagent strip can be diluted to 200000 times the human hemoglobin solution showed positive for the forensic test common in eight animal blood solution showed negative. Conclusion The prepared anti-human hemoglobin colloidal gold reagent strip can be applied to forensic tests.