论文部分内容阅读
1987年3月,从荷兰引进的唐菖蒲球茎,在Saxany品种上,选取症状为斑驳的病株,经鉴定是烟草环斑病毒侵害的结果。通过病毒寄主范围的测定、它可侵染豆科、茄科、藜科、葫芦科、番杏科中的17种植物。摩擦接种在菜豆“Pinto”、“家雀旦”;豇豆“黑种三尺”,“黑眼”,按种叶出现局部坏死斑,系统生长点坏死。大豆“猴子毛”,“晋豆84”局部坏死斑,顶枯。在苋色藜及昆诺阿藜上,接种叶局部坏死斑,无系统症状。普通烟White Buriey,按种叶局部退绿环斑,系统退绿环斑和橡叶。番杏、二青黄瓜接种叶局部退绿斑,系统花叶。病毒的致死温度为65℃,稀释终点10-~4,体外存活期6—7天(室温)。病毒粒体为等轴对称,直径约28nm。琼脂双扩散其沉淀线与标准的TRSV沉淀线完全相接,说明其抗原性相同。病毒外壳蛋白由一种蛋白亚基构成,分子量约58000道尔顿。
March 1987, imported from the Netherlands gladiolus corms, Saxany varieties in the selection of the symptoms of mottled strains, identified as a result of tobacco ring spot virus damage. Through the determination of the virus host range, it can infect legumes, Solanaceae, Chenopodiaceae, Cucurbitaceae, Aizai Branch of the 17 kinds of plants. Friction in the bean “Pinto”, “house finches”; cowpea “black three feet”, “black eyes”, according to the leaves appear local necrotic spots, system growth point necrosis. Soybean “monkey hair”, “Jin Dou 84” local necrotic spots, the top dead. In the amaranth and quinoa quinoa, inoculation leaves local necrotic spots, no systemic symptoms. Common White Smoke White Buriey, according to the local leaves back green ring spot, the system back green ring spot and oak. Apoptree apricot, two green cucumber inoculation leaves local regression green spots, system mosaic. The lethal temperature of the virus is 65 ° C, the end point of dilution is 10- to 4, and the in vitro survival period is 6-7 days (room temperature). Virus mitochondria is equiaxed symmetric, about 28nm in diameter. Agar double diffusion sedimentation line and the standard TRSV precipitation line completely connected, indicating the same antigenicity. The viral coat protein consists of a protein subunit with a molecular weight of about 58,000 daltons.